Biotechnology: Principles and Processes Chapter-Wise Test 12

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following statements about restriction enzymes is correct?

Restriction enzymes recognize specific DNA sequences and cut them.
Restriction enzymes randomly degrade DNA.
Restriction enzymes are not required for recombinant DNA technology.
Restriction enzymes only work in eukaryotic cells.
1

Restriction enzymes recognize palindromic DNA sequences and cut at specific sites.

What is the purpose of downstream processing in biotechnology?

To amplify DNA
To produce mRNA
To purify and process biotechnological products
To transfer DNA into host cells
3

Downstream processing involves purification and formulation of biotechnological products before they are marketed or used.

Which of the following statements about polymerase chain reaction (PCR) is correct?

PCR is used to amplify specific DNA sequences.
PCR does not require primers.
PCR does not involve temperature changes.
PCR cannot be used in forensic analysis.
1

PCR is a technique used to amplify specific DNA sequences using primers, DNA polymerase, and temperature cycles.

Which of the following enzymes is used to break down bacterial cell walls during DNA isolation?

Cellulase
Lysozyme
Chitinase
Ribonuclease
2

Lysozyme breaks down the peptidoglycan cell wall of bacteria to release DNA for isolation.

Which of the following statements about polymerase chain reaction (PCR) is correct?

PCR requires thermostable DNA polymerase.
PCR does not require primers.
PCR amplifies RNA instead of DNA.
PCR can only be performed at room temperature.
1

PCR requires a thermostable DNA polymerase, such as Taq polymerase, to withstand high temperatures during DNA amplification.

Which of the following statements about gel electrophoresis is incorrect?

Gel electrophoresis separates DNA fragments based on size.
Smaller DNA fragments migrate slower than larger fragments.
Agarose gel is commonly used for DNA electrophoresis.
Gel electrophoresis requires an electric field to move DNA through the gel.
2

Smaller DNA fragments migrate faster through the gel than larger fragments due to lower resistance.

Which of the following restriction enzymes was the first to be identified?

EcoRI
BamHI
Hind II
Taq polymerase
3

Hind II was the first restriction enzyme to be discovered and was used to cut DNA at specific recognition sites.

Which of the following conditions is essential for the growth of a recombinant bacterium in a bioreactor?

Sterile environment
High salt concentration
No oxygen supply
Extreme pH levels
1

A sterile environment is crucial in a bioreactor to prevent contamination and ensure the growth of only the desired recombinant bacteria.

Which of the following enzymes is NOT involved in recombinant DNA technology?

Restriction endonuclease
DNA ligase
RNA polymerase
Reverse transcriptase
3

RNA polymerase is involved in transcription, not in recombinant DNA technology.

Which of the following steps occurs first in the process of recombinant DNA technology?

Ligation of DNA into the vector
Isolation of the gene of interest
Insertion into the host organism
Amplification of recombinant DNA
2

The first step in recombinant DNA technology is isolating the gene of interest before insertion into a vector.

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