Biotechnology: Principles and Processes Chapter-Wise Test 13

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following statements about the Ti plasmid is incorrect?

The Ti plasmid is used to transfer genes into plant cells.
The Ti plasmid is derived from Agrobacterium tumefaciens.
The Ti plasmid is used in animal genetic engineering.
A disarmed Ti plasmid is used as a vector in plant biotechnology.
3

The Ti plasmid is specifically used for transferring genes into plant cells, not for animal genetic engineering.

Which feature of restriction enzymes makes them suitable for recombinant DNA technology?

They cut DNA randomly
They cut DNA at specific recognition sites
They degrade unwanted proteins
They join DNA fragments
2

Restriction enzymes cut DNA at specific recognition sequences, making them useful for precise genetic modifications.

Which of the following statements about DNA ligase is correct?

DNA ligase is used to join DNA fragments by forming phosphodiester bonds.
DNA ligase is responsible for cutting DNA at specific recognition sites.
DNA ligase is not required in recombinant DNA technology.
DNA ligase prevents DNA replication.
1

DNA ligase forms phosphodiester bonds between DNA fragments, sealing nicks in the sugar-phosphate backbone.

Which of the following statements about plasmids is correct?

Plasmids are extra-chromosomal, circular DNA molecules.
Plasmids cannot replicate independently inside a host cell.
Plasmids are always linear in structure.
Plasmids cannot be used as vectors in genetic engineering.
1

Plasmids are extra-chromosomal DNA molecules that replicate independently and are commonly used as cloning vectors.

Which of the following statements about transformation is correct?

Transformation is the process of introducing foreign DNA into bacterial cells.
Transformation always requires a viral vector.
Transformation cannot occur naturally.
Transformation is not used in genetic engineering.
1

Transformation allows bacterial cells to take up foreign DNA, either naturally or through artificial methods like electroporation.

Which of the following is NOT an application of recombinant DNA technology?

Gene therapy
Production of insulin
Fermentation of alcohol
Development of genetically modified crops
3

Fermentation of alcohol is a natural microbial process and does not necessarily involve recombinant DNA technology.

Which property of restriction enzymes makes them useful in genetic engineering?

They randomly cut DNA
They degrade unwanted genes
They recognize and cut specific DNA sequences
They always cut DNA at the same position
3

Restriction enzymes cut DNA at specific recognition sequences, which allows precise genetic modifications.

Which of the following techniques is used to insert recombinant DNA into host bacterial cells?

Electroporation
Centrifugation
Precipitation
Filtration
1

Electroporation applies an electric field to make bacterial cell membranes permeable to foreign DNA.

Which of the following is NOT a characteristic of restriction enzymes?

They recognize specific palindromic sequences
They cut DNA at specific sites
They always produce blunt ends
They are derived from bacteria
3

Restriction enzymes can produce either sticky or blunt ends, depending on the type.

What is the role of a vector in recombinant DNA technology?

To cut DNA at specific sites
To replicate foreign DNA inside a host
To amplify DNA sequences in PCR
To degrade unwanted genes
2

Vectors, such as plasmids, are used to carry and replicate foreign DNA inside host cells for cloning.

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