Biotechnology: Principles and Processes Chapter-Wise Test 14

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following is a common application of recombinant DNA technology?

Production of genetically modified crops
Formation of fossils
Separation of proteins by centrifugation
Electrolysis of water
1

Recombinant DNA technology is widely used to develop genetically modified crops with desirable traits.

What is the role of a vector in recombinant DNA technology?

To cut DNA at specific sites
To replicate the foreign DNA inside a host
To amplify DNA sequences in PCR
To degrade unwanted genes
2

Vectors, such as plasmids, carry and replicate foreign DNA within a host organism, allowing gene cloning.

Which of the following statements about genetically modified organisms (GMOs) is correct?

GMOs are created using recombinant DNA technology.
GMOs cannot be used in agriculture.
GMOs have no medical applications.
GMOs are always harmful to the environment.
1

GMOs are developed using recombinant DNA technology and have applications in agriculture, medicine, and industry.

Which of the following statements about recombinant DNA is correct?

It is formed by joining DNA from different sources
It occurs only naturally
It does not replicate inside a host
It is not used in medical applications
1

Recombinant DNA is artificially created by combining DNA fragments from different sources, allowing genetic modifications.

Which of the following statements about DNA ligase is correct?

DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase is used to cut DNA at specific sequences.
DNA ligase inhibits DNA replication.
DNA ligase degrades foreign DNA.
1

DNA ligase is an enzyme that joins DNA fragments by forming phosphodiester bonds between nucleotides.

Which of the following enzymes is used in recombinant DNA technology to synthesize complementary DNA (cDNA) from mRNA?

DNA ligase
RNA polymerase
Reverse transcriptase
Endonuclease
3

Reverse transcriptase synthesizes complementary DNA (cDNA) from an mRNA template, a key step in cloning eukaryotic genes.

Which of the following statements about Southern blotting is incorrect?

Southern blotting is used to detect specific DNA sequences.
Southern blotting does not require gel electrophoresis.
Southern blotting involves DNA hybridization.
Southern blotting is useful in genetic fingerprinting.
2

Southern blotting requires gel electrophoresis to separate DNA fragments before hybridization.

Which of the following statements about bioreactors is incorrect?

Bioreactors provide optimal conditions for microbial growth.
Bioreactors are used for large-scale production of bioproducts.
Bioreactors cannot maintain sterile conditions.
Bioreactors regulate pH, oxygen levels, and temperature.
3

Bioreactors are designed to maintain sterile conditions to prevent contamination during large-scale production.

Which of the following components is essential for maintaining sterility in bioprocess engineering?

High temperature
Sterile conditions
Antibiotic resistance genes
Use of multiple restriction enzymes
2

Maintaining sterile conditions is essential in bioprocess engineering to prevent contamination and ensure the growth of desired microbes.

Which of the following statements about Southern blotting is incorrect?

Southern blotting is used to detect specific DNA sequences.
Southern blotting involves gel electrophoresis.
Southern blotting does not involve DNA hybridization.
Southern blotting is widely used in genetic research.
3

Southern blotting relies on DNA hybridization to detect specific sequences.

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