Biotechnology: Principles and Processes Chapter-Wise Test 15

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following is an example of a vector used in plant genetic engineering?

Lambda phage
pBR322
Ti plasmid
Taq polymerase
3

The Ti plasmid from Agrobacterium tumefaciens is commonly used as a vector to transfer genes into plant cells.

Which feature of Agrobacterium tumefaciens makes it useful in genetic engineering?

It naturally infects animal cells
It carries a tumor-inducing plasmid
It produces antibiotics
It can amplify DNA in PCR
2

Agrobacterium tumefaciens naturally transfers T-DNA into plant cells, making it a useful vector in plant genetic engineering.

Which of the following is a major advantage of genetic engineering over traditional hybridization?

It allows precise introduction of desired genes
It results in random mixing of traits
It requires multiple generations to show results
It only works for asexual organisms
1

Genetic engineering allows targeted modification of genes, unlike traditional hybridization, which results in random mixing of traits.

Which method is used for large-scale production of recombinant proteins?

Microinjection
Southern blotting
Bioreactors
Northern blotting
3

Bioreactors are used for large-scale production of recombinant proteins under controlled conditions.

Which of the following statements about selectable markers is correct?

Selectable markers help distinguish transformed cells from non-transformed cells.
Selectable markers prevent bacterial growth.
Selectable markers are only found in eukaryotic cells.
Selectable markers inhibit DNA replication.
1

Selectable markers, such as antibiotic resistance genes, allow researchers to identify and isolate transformed cells.

Which of the following statements about cloning vectors is incorrect?

Cloning vectors contain an origin of replication.
Cloning vectors must have selectable markers.
Cloning vectors cannot replicate inside a host cell.
Plasmids and bacteriophages are commonly used as cloning vectors.
3

Cloning vectors contain an origin of replication, allowing them to replicate independently inside a host cell.

Which of the following vectors is used for gene transfer in plants?

pBR322
Lambda phage
Ti plasmid
pUC19
3

The Ti plasmid from Agrobacterium tumefaciens is used as a vector for gene transfer in plants.

Which enzyme is responsible for sealing nicks in the sugar-phosphate backbone of DNA?

DNA polymerase
Ligase
Helicase
Endonuclease
2

DNA ligase is responsible for sealing breaks in the sugar-phosphate backbone of DNA, making it essential in recombinant DNA technology.

Which of the following statements about genetically modified organisms (GMOs) is incorrect?

GMOs are created using recombinant DNA technology.
GMOs can be used in agriculture for pest resistance.
GMOs have no applications in medicine.
GMOs can be used to produce recombinant proteins.
3

GMOs have significant applications in medicine, including the production of insulin and vaccines.

Which of the following is NOT a component required for polymerase chain reaction (PCR)?

DNA template
Primers
Taq polymerase
Ligase
4

Ligase is not required for PCR. Instead, Taq polymerase, primers, and a DNA template are essential.

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