Biotechnology: Principles and Processes Chapter-Wise Test 19

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following methods can be used to introduce DNA into animal cells?

Electroporation
Conjugation
Transformation
Southern blotting
1

Electroporation uses an electric field to create pores in the cell membrane, allowing DNA to enter animal cells.

Which of the following methods is used to insert foreign DNA into plant cells?

Electroporation
Microinjection
Biolistics
Conjugation
3

Biolistics, or the gene gun method, introduces DNA into plant cells using high-velocity microprojectiles.

Which of the following techniques is used to separate DNA fragments?

PCR
Gel electrophoresis
Southern blotting
Western blotting
2

Gel electrophoresis separates DNA fragments based on their size using an electric field.

Which enzyme is used to join DNA fragments in recombinant DNA technology?

Restriction enzyme
DNA polymerase
DNA ligase
Helicase
3

DNA ligase is responsible for joining DNA fragments by forming phosphodiester bonds.

Which of the following statements about restriction enzymes is incorrect?

They cut DNA at specific recognition sites.
They always produce blunt ends.
They are derived from bacteria.
They recognize palindromic sequences in DNA.
2

Restriction enzymes can produce either sticky or blunt ends when cutting DNA, depending on the enzyme type.

Which of the following is NOT a feature of a cloning vector?

Origin of replication
Selectable marker
Multiple cloning sites
Exons and introns
4

Cloning vectors do not contain exons and introns; they are designed to carry and replicate inserted genes.

What is the purpose of heating the DNA sample to 94°C during PCR?

To activate DNA ligase
To denature the DNA strands
To allow restriction enzymes to cut DNA
To prevent contamination
2

Heating DNA to 94°C during PCR denatures the double-stranded DNA into single strands, allowing primers to bind in the next step.

Which of the following is used to extract and purify DNA fragments from an agarose gel?

Electroporation
Elution
Hybridization
Transformation
2

Elution is the process of extracting DNA fragments from an agarose gel for further use in recombinant DNA experiments.

Which term refers to the ability of a plasmid to replicate independently inside a host cell?

Transformation
Transduction
Autonomous replication
Conjugation
3

Autonomous replication allows plasmids to multiply independently of the host chromosome.

Which of the following statements about transformation is correct?

Transformation is the process of introducing foreign DNA into bacterial cells.
Transformation can only occur naturally and cannot be induced.
Transformation always requires viral vectors.
Transformation prevents the replication of recombinant DNA.
1

Transformation is a method used to introduce foreign DNA into bacterial cells, either naturally or through artificial methods like electroporation.

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