Biotechnology: Principles and Processes
Test 4

Correct answer Carries: 4.

Wrong Answer Carries: -1.

What is the purpose of the origin of replication (ori) in a cloning vector?

To provide antibiotic resistance
To initiate DNA replication
To cut the DNA at specific sites
To enable transformation of the host cell
2

The origin of replication (ori) is the site where DNA replication begins, allowing the plasmid to multiply inside the host cell.

Which of the following statements about restriction enzymes is correct?

They always produce blunt ends
They recognize palindromic sequences
They randomly cut DNA
They unwind the DNA helix
2

Restriction enzymes recognize specific palindromic sequences in DNA and cut at those sites.

Which of the following enzymes is used to cut DNA at specific recognition sites?

DNA polymerase
Ligase
Restriction endonuclease
Reverse transcriptase
3

Restriction endonucleases recognize specific sequences in DNA and cut at precise locations, facilitating genetic modifications.

Which of the following is the most commonly used host for cloning in recombinant DNA technology?

Streptococcus
Escherichia coli
Bacillus subtilis
Saccharomyces cerevisiae
2

Escherichia coli is commonly used as a host for cloning due to its well-studied genetics and rapid growth.

Which of the following serves as a selectable marker in cloning vectors?

Enhancers
Promoters
Antibiotic resistance genes
Silencers
3

Antibiotic resistance genes help differentiate between recombinant and non-recombinant cells in cloning experiments.

Which of the following statements about cloning vectors is correct?

Cloning vectors must contain an origin of replication.
Cloning vectors cannot carry foreign DNA.
Cloning vectors prevent DNA replication.
Cloning vectors must lack selectable markers.
1

A cloning vector must contain an origin of replication to allow it to replicate independently in a host cell.

Which of the following statements about cloning vectors is correct?

Cloning vectors contain an origin of replication.
Cloning vectors do not contain selectable markers.
Cloning vectors cannot carry foreign DNA.
Cloning vectors cannot replicate inside a host cell.
1

Cloning vectors must contain an origin of replication to allow independent replication in a host cell.

Which of the following statements about DNA ligase is incorrect?

DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase is required for recombinant DNA technology.
DNA ligase acts as a restriction enzyme.
DNA ligase is involved in DNA replication and repair.
3

DNA ligase does not act as a restriction enzyme; instead, it joins DNA fragments by sealing nicks in the sugar-phosphate backbone.

Which of the following statements about recombinant DNA technology is correct?

Recombinant DNA technology allows foreign genes to be inserted into host cells.
Recombinant DNA cannot replicate inside a host organism.
Recombinant DNA technology does not require restriction enzymes.
Recombinant DNA is always destroyed by host cells.
1

Recombinant DNA technology enables the insertion and replication of foreign genes within a host cell.

Which of the following statements about genetically modified organisms (GMOs) is incorrect?

GMOs are created using recombinant DNA technology.
GMOs can be used to produce pharmaceuticals.
GMOs have no applications in medicine.
GMOs can be used in agriculture to improve crop yield.
3

GMOs have significant applications in medicine, including the production of insulin, vaccines, and other therapeutic proteins.

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