Biotechnology: Principles and Processes
Test 5

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following best describes recombinant DNA?

DNA obtained from a single organism
DNA formed by joining fragments from different sources
DNA that undergoes mutation naturally
Unaltered chromosomal DNA
2

Recombinant DNA is artificially created by combining DNA fragments from different sources, allowing genetic modifications.

Which type of restriction enzyme makes cuts within a DNA molecule?

Exonuclease
Endonuclease
Ligase
Helicase
2

Endonucleases cut DNA at specific internal sites, unlike exonucleases, which remove nucleotides from the ends.

Which of the following is the main function of restriction endonucleases in recombinant DNA technology?

Joining DNA fragments
Cutting DNA at specific sequences
Amplifying DNA sequences
Unwinding DNA strands
2

Restriction endonucleases recognize specific DNA sequences and cut at those sites, enabling genetic modifications.

Which of the following is an essential feature of a cloning vector?

Origin of replication
Ability to degrade DNA
Lack of selectable markers
No recognition sites for restriction enzymes
1

The origin of replication allows the cloning vector to replicate independently inside the host cell.

Which of the following is a commonly used method to introduce recombinant DNA into plant cells?

Microinjection
Gene gun
Electrophoresis
Northern blotting
2

The gene gun (biolistics) method is used to introduce recombinant DNA into plant cells by shooting DNA-coated particles.

Which of the following techniques is used for separating DNA fragments based on size?

Western blotting
Southern blotting
Gel electrophoresis
PCR
3

Gel electrophoresis is used to separate DNA fragments based on size, with smaller fragments migrating faster in the gel.

Which of the following statements about recombinant DNA technology is correct?

Recombinant DNA technology allows genes from one organism to be inserted into another.
Recombinant DNA cannot replicate inside host cells.
Recombinant DNA technology does not use restriction enzymes.
Recombinant DNA is always degraded by host enzymes.
1

Recombinant DNA technology allows the insertion of foreign genes into another organism to produce desirable traits or proteins.

Which of the following statements about DNA ligase is incorrect?

DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase is used to cut DNA at specific sites.
DNA ligase is essential for recombinant DNA technology.
DNA ligase helps repair DNA during replication.
2

DNA ligase does not cut DNA; it joins DNA fragments by forming phosphodiester bonds between nucleotides.

Which of the following statements about plasmids is correct?

Plasmids are small, circular DNA molecules found in bacteria.
Plasmids are always a part of the bacterial chromosome.
Plasmids cannot be used as vectors in genetic engineering.
Plasmids cannot replicate independently.
1

Plasmids are extra-chromosomal, circular DNA molecules that can replicate independently and are often used as vectors in genetic engineering.

Which of the following statements about transformation is incorrect?

Transformation is the process of introducing foreign DNA into bacterial cells.
Transformation can be induced using calcium chloride treatment.
Transformation always requires a viral vector.
Some bacteria are naturally competent for transformation.
3

Transformation does not always require a viral vector; it can be done using chemical treatments or electroporation.

Performance Summary

Score:

Category Details
Total Attempts:
Total Skipped:
Total Wrong Answers:
Total Correct Answers:
Time Taken:
Average Time Taken per Question:
Accuracy:
0