Biotechnology: Principles and Processes Chapter-Wise Test 6

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following is NOT a feature of a good cloning vector?

Selectable marker
Multiple cloning sites
Lack of origin of replication
Ability to replicate independently
3

A good cloning vector must have an origin of replication to ensure its multiplication inside the host cell.

Which of the following statements about restriction enzymes is incorrect?

Restriction enzymes recognize specific DNA sequences.
All restriction enzymes cut DNA randomly.
Some restriction enzymes produce sticky ends.
Restriction enzymes are derived from bacteria.
2

Restriction enzymes do not cut DNA randomly; they recognize specific sequences and cut at precise locations.

Which of the following statements about transformation is correct?

Transformation is the uptake of foreign DNA by bacterial cells.
Transformation requires viral infection.
Transformation cannot occur naturally.
Transformation does not involve recombinant DNA technology.
1

Transformation is the process of introducing foreign DNA into bacterial cells, either naturally or artificially.

Which of the following is an advantage of recombinant DNA technology?

Production of genetically modified crops
Increase in fossil fuel consumption
Degradation of natural enzymes
Prevention of DNA replication
1

Recombinant DNA technology is widely used to develop genetically modified crops with improved traits.

Which of the following statements about DNA ligase is correct?

DNA ligase joins DNA fragments by forming hydrogen bonds.
DNA ligase is not involved in recombinant DNA technology.
DNA ligase is responsible for sealing gaps in the sugar-phosphate backbone.
DNA ligase cuts DNA at specific recognition sites.
3

DNA ligase joins DNA fragments by forming phosphodiester bonds, sealing gaps in the sugar-phosphate backbone.

Which of the following is NOT an application of recombinant DNA technology?

Gene therapy
Production of insulin
Fermentation of alcohol
Development of genetically modified crops
3

Fermentation of alcohol is a natural microbial process and does not necessarily involve recombinant DNA technology.

Which of the following statements about cloning vectors is incorrect?

Cloning vectors contain an origin of replication.
Cloning vectors must have selectable markers.
Cloning vectors cannot replicate inside a host cell.
Plasmids and bacteriophages are common cloning vectors.
3

Cloning vectors contain an origin of replication, allowing them to replicate independently inside a host cell.

Which of the following statements about gel electrophoresis is correct?

Gel electrophoresis separates DNA fragments based on charge.
Smaller DNA fragments migrate faster through the gel than larger fragments.
Gel electrophoresis does not require an electric field.
All DNA fragments migrate at the same speed.
2

In gel electrophoresis, DNA fragments are separated based on size, with smaller fragments migrating faster than larger ones.

What is the function of restriction enzymes in bacteria?

To replicate DNA
To protect against viral infections
To facilitate protein synthesis
To help in nutrient absorption
2

Restriction enzymes in bacteria act as a defense mechanism by cutting foreign viral DNA to prevent infection.

Which of the following is NOT a component of a cloning vector?

Origin of replication
Selectable marker
Promoter region
Ribosome
4

Ribosomes are involved in protein synthesis, not in DNA replication or vector construction.

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