Biotechnology: Principles and Processes Chapter-Wise Test 7

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which enzyme is used to join DNA fragments during recombinant DNA technology?

Restriction enzyme
DNA polymerase
DNA ligase
Helicase
3

DNA ligase is responsible for joining DNA fragments by forming phosphodiester bonds.

Which method uses gold or tungsten particles to introduce foreign DNA into plant cells?

Microinjection
Biolistics
Electroporation
Liposome-mediated transfer
2

Biolistics, also known as the gene gun method, delivers DNA into plant cells using high-velocity metal particles.

Which of the following statements about cloning vectors is incorrect?

Cloning vectors contain an origin of replication.
Cloning vectors lack selectable markers.
Cloning vectors are used to carry foreign DNA.
Cloning vectors can replicate independently inside host cells.
2

Cloning vectors contain selectable markers, such as antibiotic resistance genes, to help identify transformed cells.

Which of the following statements about selectable markers is incorrect?

Selectable markers help distinguish transformed cells.
Selectable markers include antibiotic resistance genes.
Selectable markers inhibit bacterial growth.
Selectable markers help identify recombinant plasmids.
3

Selectable markers do not inhibit bacterial growth but rather help distinguish transformed from non-transformed cells.

Which of the following statements about recombinant DNA technology is correct?

It involves the random mixing of genes
It allows insertion of specific genes into a host
It uses only bacterial cells as hosts
It does not involve any enzymes
2

Recombinant DNA technology enables the insertion of specific genes into host organisms for desired traits.

Which of the following is a key advantage of PCR over traditional cloning methods?

PCR requires living cells
PCR can amplify DNA in hours
PCR introduces fewer mutations
PCR does not require primers
2

PCR is a rapid method for amplifying DNA sequences within hours, unlike traditional cloning, which takes days or weeks.

Which of the following statements about DNA ligase is correct?

DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase is used to cut DNA at specific sites.
DNA ligase prevents DNA replication.
DNA ligase is not involved in recombinant DNA technology.
1

DNA ligase seals nicks in the sugar-phosphate backbone by forming phosphodiester bonds between DNA fragments.

Which of the following statements about PCR is incorrect?

PCR is used to amplify DNA sequences.
PCR requires thermostable DNA polymerase.
PCR involves three main steps: denaturation, annealing, and extension.
PCR is used to cut DNA at specific sites.
4

PCR is not used to cut DNA; it is used to amplify specific DNA sequences.

Which step in recombinant DNA technology involves making multiple copies of a gene?

Isolation of DNA
Insertion of DNA into a vector
Gene amplification using PCR
Selection of recombinant cells
3

Polymerase Chain Reaction (PCR) is used to amplify specific DNA sequences, producing multiple copies of a gene.

Which of the following statements about restriction enzymes is incorrect?

Restriction enzymes recognize specific DNA sequences.
All restriction enzymes produce sticky ends.
Restriction enzymes are used in recombinant DNA technology.
Restriction enzymes are derived from bacteria.
2

Not all restriction enzymes produce sticky ends; some generate blunt ends instead.

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