Biotechnology: Principles and Processes Chapter-Wise Test 8

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following statements about Southern blotting is correct?

Southern blotting is used to detect specific DNA sequences.
Southern blotting is used for protein detection.
Southern blotting does not involve gel electrophoresis.
Southern blotting is not useful for forensic science.
1

Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample.

Which of the following statements about DNA ligase is correct?

DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase cuts DNA at specific recognition sites.
DNA ligase prevents DNA replication.
DNA ligase is not used in recombinant DNA technology.
1

DNA ligase forms phosphodiester bonds between DNA fragments, sealing nicks in the sugar-phosphate backbone.

Which of the following is NOT a core technique of modern biotechnology?

Genetic engineering
Bioprocess engineering
Selective breeding
Recombinant DNA technology
3

Selective breeding is a traditional technique, while genetic engineering, bioprocess engineering, and recombinant DNA technology are core to modern biotechnology.

Which of the following statements about recombinant DNA technology is incorrect?

Recombinant DNA technology allows for the insertion of foreign genes into host cells.
Recombinant DNA cannot be replicated inside host cells.
Vectors are commonly used in recombinant DNA technology.
Restriction enzymes are essential for recombinant DNA technology.
2

Recombinant DNA can replicate inside host cells when inserted into a suitable vector with an origin of replication.

Which of the following is a method for direct gene transfer into animal cells?

Biolistics
Microinjection
Electroporation
Conjugation
2

Microinjection is a technique used to introduce foreign DNA directly into the nucleus of animal cells.

Which of the following is a feature of a good cloning vector?

It should have multiple origins of replication
It should have a selectable marker
It should not replicate in the host cell
It should be unstable
2

A good cloning vector must have a selectable marker to help identify successfully transformed cells.

Which of the following methods is used to separate DNA fragments based on size?

Southern blotting
Northern blotting
Gel electrophoresis
Polymerase chain reaction
3

Gel electrophoresis separates DNA fragments based on size, with smaller fragments migrating faster through the agarose gel.

Which of the following statements about polymerase chain reaction (PCR) is correct?

PCR is used to amplify DNA sequences.
PCR uses DNA ligase to replicate DNA.
PCR cannot be performed using thermostable enzymes.
PCR does not require primers.
1

PCR is a technique used to amplify specific DNA sequences using thermostable enzymes like Taq polymerase and primers.

Which step in recombinant DNA technology involves making multiple copies of a gene?

Isolation of DNA
Insertion of DNA into a vector
Gene amplification using PCR
Selection of recombinant cells
3

Polymerase Chain Reaction (PCR) is used to amplify specific DNA sequences, producing multiple copies of a gene.

Which of the following components is essential for the polymerase chain reaction (PCR)?

Ligase
Taq polymerase
Restriction enzyme
Reverse transcriptase
2

Taq polymerase, derived from Thermus aquaticus, is a thermostable enzyme used in PCR to amplify DNA.

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