Biotechnology: Principles and Processes Chapter-Wise Test 9

Correct answer Carries: 4.

Wrong Answer Carries: -1.

What is the purpose of gel electrophoresis in recombinant DNA technology?

To separate DNA fragments based on size
To amplify DNA sequences
To replicate plasmid DNA
To transform bacterial cells
1

Gel electrophoresis is used to separate DNA fragments based on size, allowing analysis and purification of DNA samples.

What is the function of DNA ligase in recombinant DNA technology?

To cut DNA at specific sites
To unwind the DNA helix
To join DNA fragments
To degrade unwanted nucleotides
3

DNA ligase joins DNA fragments by forming phosphodiester bonds, allowing successful recombination.

Which of the following is a crucial step before inserting recombinant DNA into a host organism?

DNA sequencing
Isolation of the desired gene
Protein synthesis
Southern blotting
2

Before inserting recombinant DNA, the desired gene must be isolated from the source organism.

Which of the following statements about gel electrophoresis is incorrect?

It separates DNA fragments based on size.
DNA fragments move toward the negative electrode.
Agarose gel is commonly used for DNA electrophoresis.
Smaller DNA fragments migrate faster than larger fragments.
2

DNA is negatively charged and moves toward the positive electrode (anode) in gel electrophoresis, not the negative electrode.

Which of the following statements about restriction enzymes is incorrect?

Restriction enzymes recognize specific DNA sequences and cut them.
Restriction enzymes are naturally found in bacteria.
All restriction enzymes produce sticky ends.
Restriction enzymes are used in recombinant DNA technology.
3

Not all restriction enzymes produce sticky ends; some generate blunt ends when cutting DNA.

Which of the following statements about plasmids is TRUE?

They are linear DNA molecules
They replicate independently of the host chromosome
They do not contain any genes
They are only found in eukaryotic cells
2

Plasmids are circular, extra-chromosomal DNA molecules that replicate independently of the host genome.

Which of the following statements about cloning vectors is incorrect?

Cloning vectors contain an origin of replication.
Cloning vectors must have selectable markers.
Cloning vectors cannot replicate inside a host cell.
Plasmids and bacteriophages are common cloning vectors.
3

Cloning vectors can replicate inside a host cell due to the presence of an origin of replication.

Which of the following statements about gel electrophoresis is incorrect?

Gel electrophoresis separates DNA fragments based on size.
Smaller DNA fragments move faster than larger ones.
DNA moves toward the negative electrode during electrophoresis.
Gel electrophoresis requires an electric field.
3

DNA is negatively charged and moves toward the positive electrode (anode) in gel electrophoresis.

Which of the following statements about vectors in genetic engineering is incorrect?

Vectors help in transferring foreign DNA into host cells.
Vectors must contain an origin of replication.
Vectors cannot contain selectable markers.
Plasmids and bacteriophages are commonly used as vectors.
3

Vectors often contain selectable markers, such as antibiotic resistance genes, to help identify transformed cells.

Which of the following statements about Southern blotting is correct?

Southern blotting is used to detect specific DNA sequences.
Southern blotting is used to detect proteins.
Southern blotting does not require gel electrophoresis.
Southern blotting is not useful in forensic science.
1

Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample.

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