Biotechnology and its Applications Chapter-Wise Test 10

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following statements about gene therapy is incorrect?

Gene therapy is used to replace defective genes with functional ones.
Gene therapy is used for treating genetic disorders like ADA deficiency.
Gene therapy is performed at the protein level.
Gene therapy can involve viral vectors for gene delivery.
3

Gene therapy is performed at the genetic (DNA) level, not at the protein level.

Which molecule is used as a probe in molecular diagnostics for detecting specific DNA sequences?

mRNA
DNA probe
tRNA
rRNA
2

DNA probes are used in molecular diagnostics to detect specific DNA sequences by hybridization.

Which technique is used for early detection of genetic disorders by amplifying specific DNA sequences?

ELISA
PCR
Gel electrophoresis
Southern blotting
2

PCR (Polymerase Chain Reaction) is used for early detection of genetic disorders by amplifying specific DNA sequences.

Which plant part is used in somatic hybridization after enzymatic removal of cell walls?

Protoplast
Callus
Meristem
Embryo
1

Protoplasts are used in somatic hybridization after the enzymatic removal of cell walls.

Which enzyme is responsible for removing the C peptide during the maturation of insulin?

Peptidase
Protease
Endonuclease
Exonuclease
2

Protease removes the C peptide during the maturation of proinsulin into active insulin.

Which regulatory body in India is responsible for approving genetically modified organisms?

ICMR
GEAC
DBT
CSIR
2

The Genetic Engineering Approval Committee (GEAC) is responsible for approving and regulating genetically modified organisms in India.

Which of the following is incorrect about Golden Rice?

Golden Rice is genetically modified to produce beta-carotene.
Golden Rice helps reduce vitamin A deficiency.
Golden Rice is produced naturally without genetic modification.
Golden Rice was developed using genetic engineering techniques.
3

Golden Rice is genetically modified to produce beta-carotene and is not produced naturally without genetic modification.

Which protein crystals produced by Bacillus thuringiensis kill insect pests?

Toxin B
Cry protein
Bt protein
Lysin
2

Cry proteins produced by Bacillus thuringiensis form crystals that are toxic to insect pests.

Which protein produced by Bacillus thuringiensis is toxic to insect larvae?

Bt protein
Cry protein
Protease
Collagen
2

Cry protein produced by Bacillus thuringiensis is toxic to insect larvae and is used in pest-resistant crops.

Which enzyme is used to cut DNA at specific sites during genetic manipulation?

Ligase
Endonuclease
Reverse transcriptase
DNA polymerase
2

Endonuclease enzymes cut DNA at specific recognition sites during genetic manipulation.

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