Biotechnology and its Applications Chapter-Wise Test 14

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which enzyme deficiency is treated by gene therapy using retroviral vectors?

Hexosaminidase A
Phenylalanine hydroxylase
Adenosine deaminase
Tyrosinase
3

Gene therapy is used to treat adenosine deaminase (ADA) deficiency using retroviral vectors.

Which crop faced biopiracy issues when patented by a foreign company?

Wheat
Basmati rice
Sugarcane
Tea
2

Basmati rice from India faced biopiracy issues when a foreign company patented its varieties.

Which biotechnological method is used to obtain virus-free plants?

Somatic hybridization
RNA interference
Meristem culture
Protoplast fusion
3

Meristem culture is used to produce virus-free plants since meristems are usually free from viruses.

Which of the following statements is incorrect about somatic hybridization?

Somatic hybridization involves the fusion of protoplasts.
Somatic hybridization is a method of asexual reproduction.
Somatic hybridization produces genetically identical clones.
Pomato is an example of somatic hybridization.
3

Somatic hybridization produces hybrid plants with traits from both parent species, not genetically identical clones.

Which of the following is correct about recombinant DNA technology?

Recombinant DNA technology is used to produce identical RNA strands.
Recombinant DNA is formed by combining DNA from different sources.
Recombinant DNA technology is unrelated to gene cloning.
Recombinant DNA technology prevents DNA replication.
2

Recombinant DNA technology involves combining DNA from different sources to form recombinant DNA for various applications, including gene cloning.

Which of the following statements is correct about polymerase chain reaction (PCR)?

PCR is used for amplifying DNA sequences.
PCR does not require primers.
PCR is a method of protein synthesis.
PCR is performed without temperature changes.
1

PCR is a technique used to amplify specific DNA sequences through cycles of denaturation, annealing, and extension.

Which technology is used for large-scale production of genetically identical plants?

Tissue culture
Mutation breeding
Genetic mapping
RNA interference
1

Tissue culture is used for large-scale production of genetically identical plants through micropropagation.

Which of the following is correct about polymerase chain reaction (PCR)?

PCR uses RNA primers for DNA amplification.
PCR does not require a DNA template.
PCR amplifies DNA through repeated cycles of denaturation, annealing, and extension.
PCR is not used in genetic research.
3

PCR amplifies specific DNA sequences through repeated cycles of denaturation, annealing of primers, and extension by DNA polymerase.

Which of the following is incorrect about molecular diagnostics?

Molecular diagnostics uses PCR to detect genetic material.
Molecular diagnostics can identify genetic mutations.
Molecular diagnostics relies solely on visual inspection.
Molecular diagnostics is used in disease management.
3

Molecular diagnostics uses advanced techniques like PCR, DNA probes, and sequencing, not just visual inspection, for accurate detection.

Which component of the nutrient medium provides vitamins essential for plant tissue culture?

Sucrose
Inorganic salts
Vitamins
Amino acids
3

Vitamins in the nutrient medium are essential for plant cell growth and differentiation in tissue culture.

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