Biotechnology and its Applications Chapter-Wise Test 15

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following statements is incorrect about gene therapy?

Gene therapy involves replacing defective genes with functional ones.
Gene therapy is used for treating genetic disorders like ADA deficiency.
Gene therapy is primarily performed at the protein level.
Gene therapy can use viral vectors for gene delivery.
3

Gene therapy involves manipulating genes at the DNA level, not at the protein level.

Which biotechnological application uses retroviral vectors to deliver genes into cells?

Micropropagation
Somatic hybridization
Gene therapy
ELISA
3

Gene therapy uses retroviral vectors to deliver therapeutic genes into cells to treat genetic disorders.

Which of the following is a feature of genetically modified crops?

Decreased resistance to pests
Increased need for chemical pesticides
Tolerance to abiotic stresses
Reduced efficiency of mineral absorption
3

GM crops are designed to be tolerant to environmental stresses such as drought and extreme temperatures.

Which of the following is correct about restriction endonucleases?

Restriction endonucleases are used to degrade proteins.
Restriction endonucleases cut DNA at specific recognition sites.
Restriction endonucleases are involved in protein synthesis.
Restriction endonucleases synthesize RNA molecules.
2

Restriction endonucleases cut DNA at specific recognition sites, which is essential in recombinant DNA technology.

Which enzyme is used to cut DNA molecules at specific recognition sites?

DNA polymerase
Ligase
Restriction enzyme
Helicase
3

Restriction enzymes, also called molecular scissors, cut DNA at specific recognition sites.

Which molecule triggers RNA interference to silence gene expression?

mRNA
tRNA
dsRNA
rRNA
3

Double-stranded RNA (dsRNA) triggers RNA interference, leading to the silencing of specific gene expression.

Which of the following is correct about Golden Rice?

Golden Rice is genetically modified to produce high protein content.
Golden Rice is genetically modified to produce beta-carotene.
Golden Rice is used for nitrogen fixation in soil.
Golden Rice is resistant to fungal infections.
2

Golden Rice is genetically modified to produce beta-carotene, which helps combat vitamin A deficiency.

Which molecule is used as a probe in molecular diagnostics to detect specific DNA sequences?

tRNA
mRNA
DNA probe
rRNA
3

DNA probes are used in molecular diagnostics to detect specific DNA sequences by hybridization.

Which of the following is incorrect about RNA interference (RNAi)?

RNAi silences specific gene expression.
RNAi involves double-stranded RNA molecules.
RNAi enhances gene expression.
RNAi is used to develop pest-resistant crops.
3

RNA interference is used to silence, not enhance, specific gene expression in organisms.

What is the function of selectable marker genes in recombinant DNA technology?

Enhance gene expression
Identify transformed cells
Silence unwanted genes
Facilitate DNA replication
2

Selectable marker genes help identify transformed cells by providing resistance to antibiotics or other selectable agents.

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