Biotechnology and its Applications Chapter-Wise Test 19

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which technique is used to introduce foreign DNA into animal cells using fine glass needles?

Electroporation
Microinjection
Gene gun
Lipofection
2

Microinjection is used to introduce foreign DNA into animal cells using fine glass needles under a microscope.

Which method is used to isolate naked protoplasts from plant cells?

Mechanical grinding
Enzymatic digestion
Electroporation
Centrifugation
2

Enzymatic digestion is used to remove the cell wall and isolate naked protoplasts from plant cells.

Which organism's toxin gene is used in genetically modified crops for pest resistance?

Escherichia coli
Bacillus thuringiensis
Agrobacterium tumefaciens
Saccharomyces cerevisiae
2

Bacillus thuringiensis toxin gene is used in GM crops to provide resistance against insect pests.

Which of the following is correct about the Genetic Engineering Approval Committee (GEAC)?

GEAC regulates the use of genetically modified organisms in India.
GEAC approves traditional plant breeding methods.
GEAC regulates pesticide usage in agriculture.
GEAC is an international body for genetic research.
1

The Genetic Engineering Approval Committee (GEAC) regulates the use, approval, and safety of genetically modified organisms in India.

Which genetically modified crop is resistant to insect pests due to the expression of cry genes?

Golden rice
Bt cotton
Hybrid maize
Tomato
2

Bacillus thuringiensis (Bt) cotton expresses cry genes, providing resistance to insect pests like bollworms.

Which nutrient component in tissue culture media provides amino acids for plant growth?

Sucrose
Vitamins
Amino acid supplements
Inorganic salts
3

Amino acid supplements in tissue culture media provide essential amino acids required for plant cell growth.

Which disease is treated using gene therapy with the insertion of ADA gene?

Cystic fibrosis
Sickle cell anemia
Adenosine deaminase deficiency
Hemophilia
3

Gene therapy is used to treat adenosine deaminase (ADA) deficiency by inserting a functional ADA gene.

Which protein produced by transgenic animals is used to treat cystic fibrosis?

Interferon
Alpha-1-antitrypsin
Insulin
Growth hormone
2

Alpha-1-antitrypsin produced by transgenic animals is used in the treatment of cystic fibrosis.

What is the purpose of using genetically modified bacteria in insulin production?

To increase bacterial resistance
To produce human insulin
To promote fermentation
To enhance bacterial growth
2

Genetically modified bacteria such as E. coli are used to produce human insulin through recombinant DNA technology.

Which technique is used for the early detection of HIV in suspected patients?

ELISA
PCR
Northern blotting
Chromatography
2

PCR is used to amplify and detect even small amounts of viral DNA, making it suitable for early HIV detection.

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