Biotechnology and Its Applications
Test 2

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which method is used to produce virus-free plants?

Micropropagation
Grafting
Genetic engineering
Tissue culture of meristem
4

The meristem is usually virus-free and can be cultured to produce virus-free plants.

What is the role of cryIAb gene in genetically modified crops?

Resistance to nematodes
Resistance to corn borer
Increased vitamin A production
Drought resistance
2

The cryIAb gene provides resistance to the corn borer pest in genetically modified crops.

Which type of RNA is involved in the RNA interference mechanism for gene silencing?

mRNA
tRNA
rRNA
dsRNA
4

Double-stranded RNA (dsRNA) is involved in RNA interference, leading to the silencing of specific genes.

Which crop is genetically modified with cryIIAb gene for resistance to pests?

Tomato
Cotton
Rice
Potato
2

Cotton is genetically modified with cryIIAb gene for resistance to bollworms.

What is the function of GEAC in India?

Approving GM crop exports
Regulating research and release of GMOs
Providing agricultural subsidies
Issuing patents for GMOs
2

The Genetic Engineering Approval Committee (GEAC) regulates the research, development, and release of genetically modified organisms in India.

Which vector is commonly used in gene therapy for transferring therapeutic genes?

Plasmid
Retrovirus
Cosmid
Phagemid
2

Retrovirus is commonly used as a vector in gene therapy to transfer therapeutic genes into host cells.

Which plant was used to demonstrate RNA interference for nematode resistance?

Potato
Tomato
Tobacco
Maize
3

Tobacco plants were genetically modified using RNA interference to demonstrate resistance against nematodes.

Which of the following is incorrect regarding RNA interference (RNAi)?

RNA interference involves double-stranded RNA.
RNAi silences specific gene expression.
RNAi is used to enhance protein synthesis in cells.
RNAi is employed to develop pest-resistant crops.
3

RNA interference is used to silence specific gene expression, not to enhance protein synthesis.

Which of the following statements about vectors in genetic engineering is correct?

Vectors are RNA molecules that carry genes for replication.
Vectors are DNA molecules used to transfer foreign genes into host cells.
Vectors are enzymes used to cut DNA.
Vectors are lipids used in cell membrane synthesis.
2

Vectors are DNA molecules, such as plasmids, used to transfer foreign genes into host cells in genetic engineering.

Which of the following is incorrect about Bt cotton?

Bt cotton is genetically modified to produce Cry proteins.
Bt cotton is resistant to insect pests like bollworms.
Bt cotton improves soil fertility naturally.
Bt cotton reduces the need for chemical pesticides.
3

Bt cotton does not improve soil fertility naturally; it is engineered to produce insecticidal proteins for pest resistance.

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