Biotechnology and its Applications Chapter-Wise Test 20

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which molecular technique is used to amplify DNA fragments in vitro?

Southern blotting
Polymerase Chain Reaction (PCR)
Gene cloning
Microarray
2

Polymerase Chain Reaction (PCR) is used to amplify specific DNA fragments in vitro for various genetic studies.

What is the main advantage of Golden Rice over conventional rice?

Increased yield
Resistance to pests
High vitamin A content
Drought tolerance
3

Golden Rice is genetically modified to have high vitamin A content, addressing vitamin A deficiency.

Which enzyme is used to cut and remove specific DNA fragments during genetic engineering?

DNA polymerase
Ligase
Restriction endonuclease
Helicase
3

Restriction endonucleases are enzymes used to cut DNA at specific recognition sites in genetic engineering.

Which crop is genetically modified to produce insecticidal proteins from Bacillus thuringiensis?

Golden rice
Bt cotton
Tomato
Soybean
2

Bacillus thuringiensis (Bt) cotton is genetically modified to produce insecticidal proteins that kill insect pests.

Which plant was genetically modified using RNA interference to combat nematode infection?

Tomato
Potato
Tobacco
Rice
3

Tobacco plants were genetically modified using RNA interference to combat nematode infection.

What does the term 'biopiracy' refer to?

Illegal trade of wildlife
Unauthorized use of biological resources
Overuse of fertilizers
Illegal genetic modifications
2

Biopiracy refers to the unauthorized exploitation of biological resources without proper compensation.

Which transgenic animal was the first to produce human protein-enriched milk?

Rosie the cow
Dolly the sheep
Oncomouse
Zebrafish
1

Rosie the transgenic cow produced milk enriched with human alpha-lactalbumin for better infant nutrition.

Which DNA amplification technique is widely used in molecular biology for gene analysis?

Northern blotting
PCR
ELISA
Microarray
2

PCR (Polymerase Chain Reaction) is widely used in molecular biology for amplifying specific DNA sequences.

Which technique is used to introduce foreign genes into plant cells using Agrobacterium?

Microinjection
Electroporation
Gene gun
Agrobacterium-mediated transfer
4

Agrobacterium-mediated transfer is commonly used to introduce foreign genes into plant cells using Ti plasmid vectors.

Which bacterium is used as a vector in the production of pest-resistant plants using RNAi?

Bacillus thuringiensis
Agrobacterium tumefaciens
Escherichia coli
Pseudomonas putida
2

Agrobacterium tumefaciens is used as a vector to introduce genes for pest resistance in plants through RNAi.

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