Biotechnology and its Applications Chapter-Wise Test 22

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which method is used to introduce foreign DNA into plant cells using high-velocity microparticles?

Microinjection
Electroporation
Gene gun
Agrobacterium-mediated transfer
3

The gene gun method introduces foreign DNA into plant cells by shooting high-velocity microparticles coated with DNA.

Which technique uses complementary DNA probes to detect specific DNA sequences?

PCR
Southern blotting
ELISA
Gene cloning
2

Southern blotting uses complementary DNA probes to detect specific DNA sequences through hybridization.

Which of the following statements is true about Golden Rice?

Golden Rice is genetically modified to produce high protein content.
Golden Rice is enriched with beta-carotene, a precursor of vitamin A.
Golden Rice is used to treat diabetes.
Golden Rice is resistant to viral infections.
2

Golden Rice is genetically modified to produce beta-carotene, which is converted into vitamin A in the body, addressing vitamin A deficiency.

Which biotechnological method is used for early diagnosis of genetic disorders?

Micropropagation
PCR
Gene editing
ELISA
2

PCR (Polymerase Chain Reaction) is used for early diagnosis of genetic disorders by amplifying specific DNA sequences.

What is the major ethical concern associated with genetically modified organisms?

Increased food production
Environmental and ecological risks
Enhanced nutrient content
Reduced agricultural costs
2

Genetic modification may have unpredictable ecological consequences, raising ethical concerns.

Which of the following statements is incorrect regarding gene therapy?

Gene therapy corrects defective genes by inserting functional genes.
Retroviruses are commonly used as vectors in gene therapy.
Gene therapy is only used for bacterial infections.
Gene therapy is used for treating genetic disorders like ADA deficiency.
3

Gene therapy is not limited to bacterial infections; it is primarily used to treat genetic disorders by correcting defective genes.

Which plant tissue culture technique is used to produce virus-free plants?

Callus culture
Protoplast fusion
Meristem culture
Somatic hybridization
3

Meristem culture is used to produce virus-free plants because meristematic tissues are usually virus-free.

Which genetically modified crop provides resistance to bollworm insects?

Bt cotton
Golden rice
Hybrid maize
Flavr Savr tomato
1

Bacillus thuringiensis (Bt) cotton is genetically modified to provide resistance against bollworm insects.

Which technique uses double-stranded RNA to silence specific genes in pests?

Gene editing
RNA interference
Somatic hybridization
Micropropagation
2

RNA interference (RNAi) uses double-stranded RNA to silence specific genes in pests, preventing their growth.

Which company first produced genetically engineered human insulin?

Genentech
Eli Lilly
Pfizer
Roche
2

Eli Lilly produced the first genetically engineered human insulin using E. coli in 1983.

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