Biotechnology and its Applications Chapter-Wise Test 23

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following is correct about RNA interference (RNAi)?

RNAi silences specific gene expression using double-stranded RNA.
RNAi enhances gene expression in all organisms.
RNAi is used for synthesizing DNA molecules.
RNAi prevents DNA replication in bacteria.
1

RNA interference (RNAi) is a biological process that silences specific gene expression using double-stranded RNA molecules.

What is the function of Bt toxin in genetically modified crops?

Increase nitrogen fixation
Provide resistance against insects
Enhance photosynthesis
Improve seed germination
2

Bt toxin, derived from Bacillus thuringiensis, protects plants by killing insect pests.

Which of the following statements is incorrect about PCR?

PCR is used to amplify specific DNA sequences.
PCR requires DNA primers for amplification.
PCR synthesizes amino acids for protein production.
PCR is used in molecular diagnostics.
3

PCR is used to amplify DNA sequences, not for synthesizing amino acids for protein production.

Which regulatory body in India oversees research and safety of genetically modified organisms?

ICMR
GEAC
CSIR
ICAR
2

The Genetic Engineering Approval Committee (GEAC) oversees research, approval, and safety of genetically modified organisms in India.

Which law in India addresses issues of biopiracy and patenting?

Indian Biodiversity Act
Indian Patents Bill
Environment Protection Act
Wildlife Protection Act
2

The Indian Patents Bill addresses biopiracy and ensures fair compensation and protection of traditional knowledge.

Which of the following is correct about gene therapy?

Gene therapy introduces harmful mutations intentionally.
Gene therapy is used to replace defective genes with functional ones.
Gene therapy is unrelated to genetic disorders.
Gene therapy prevents protein synthesis in cells.
2

Gene therapy is a technique used to replace defective genes with functional ones to treat genetic disorders.

Which vector is commonly used in plant genetic engineering for gene transfer?

Retrovirus
Ti plasmid of Agrobacterium
Lambda phage
Cosmid
2

Ti plasmid of Agrobacterium is commonly used as a vector in plant genetic engineering for gene transfer.

Which biotechnological tool is used to amplify specific DNA sequences in vitro?

Gel electrophoresis
Polymerase Chain Reaction (PCR)
ELISA
Southern blotting
2

Polymerase Chain Reaction (PCR) is used to amplify specific DNA sequences in vitro.

Which of the following statements is correct about restriction enzymes?

Restriction enzymes degrade mRNA molecules.
Restriction enzymes cut DNA at specific recognition sites.
Restriction enzymes are used to join DNA fragments.
Restriction enzymes synthesize proteins from DNA.
2

Restriction enzymes act as molecular scissors that cut DNA at specific recognition sequences.

Which molecule is produced by transgenic sheep for treating emphysema?

Human insulin
Alpha-1-antitrypsin
Human growth hormone
Interferon
2

Transgenic sheep produce alpha-1-antitrypsin, a protein used to treat emphysema.

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