Biotechnology and its Applications Chapter-Wise Test 24

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which molecule is produced during Bt toxin activation in the insect gut?

Inactive protoxin
Active toxin
DNA polymerase
mRNA
2

Bt toxin is activated in the alkaline environment of the insect gut, converting it from an inactive protoxin to an active toxin.

Which of the following statements about transgenic plants is correct?

Transgenic plants are produced by crossing different species naturally.
Transgenic plants contain genes from unrelated organisms introduced artificially.
Transgenic plants are only used for ornamental purposes.
Transgenic plants cannot be used for disease resistance.
2

Transgenic plants are genetically modified to contain genes from unrelated organisms introduced through biotechnology for traits like pest resistance and improved yield.

What is the significance of auxins in plant tissue culture?

Induces flowering
Promotes root formation
Enhances seed dormancy
Inhibits cell division
2

Auxins promote root formation in plant tissue culture.

What is the primary purpose of gene therapy?

To enhance plant growth
To correct genetic defects
To produce antibiotics
To increase crop yield
2

Gene therapy involves correcting genetic defects by introducing functional genes into cells.

Which of the following statements about the Genetic Engineering Approval Committee (GEAC) is correct?

GEAC regulates traditional plant breeding methods.
GEAC is responsible for approving and regulating genetically modified organisms in India.
GEAC is an international organization for genetic research.
GEAC only oversees the use of antibiotics in agriculture.
2

The Genetic Engineering Approval Committee (GEAC) is responsible for the approval, regulation, and safety of genetically modified organisms in India.

What is the primary aim of using RNA interference in genetically modified crops?

Enhancing nutrient uptake
Silencing specific genes in pests
Increasing photosynthesis
Promoting plant growth hormones
2

RNA interference is used to silence specific genes in pests, preventing them from causing harm to the crops.

Which of the following is true about restriction enzymes?

Restriction enzymes synthesize RNA from DNA.
Restriction enzymes cut DNA at specific sites.
Restriction enzymes are involved in protein synthesis.
Restriction enzymes are only found in plants.
2

Restriction enzymes are molecular scissors that cut DNA at specific recognition sites, which is essential for genetic engineering.

Which of the following is incorrect about plasmids in genetic engineering?

Plasmids are circular DNA molecules.
Plasmids replicate independently in host cells.
Plasmids are used as vectors to transfer genes.
Plasmids encode ribosomal RNA in humans.
4

Plasmids do not encode ribosomal RNA in humans; they are used as vectors in genetic engineering.

Which of the following is correct about somatic hybridization?

Somatic hybridization involves the fusion of whole plant cells.
Somatic hybridization fuses protoplasts from different species.
Somatic hybridization is the same as sexual reproduction.
Somatic hybridization is unrelated to plant biotechnology.
2

Somatic hybridization involves the fusion of protoplasts from different plant species to create hybrid plants with combined traits.

Which method allows the identification of mutated genes using radioactive probes?

ELISA
Southern blotting
PCR
Autoradiography
4

Autoradiography detects mutated genes by using radioactive probes that hybridize with complementary DNA.

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