Biotechnology and Its Applications
Test 3

Correct answer Carries: 4.

Wrong Answer Carries: -1.

What is totipotency in plant tissue culture?

Ability to produce roots only
Ability to regenerate a whole plant from a single cell
Ability to produce seeds from leaves
Ability to enhance photosynthesis
2

Totipotency is the ability of a single cell or explant to regenerate into a whole plant under sterile conditions.

What does the term 'somaclones' refer to?

Plants produced through hybridization
Genetically identical plants produced via tissue culture
Plants with random mutations
Plants produced through genetic engineering
2

Somaclones are genetically identical plants produced through micropropagation in tissue culture.

What is the key benefit of Bt cotton in agriculture?

Increased water absorption
Resistance to bollworm pests
Enhanced seed germination
Higher nutrient content
2

Bt cotton contains cry genes from Bacillus thuringiensis, providing resistance against bollworm pests.

Which Indian law prevents the unauthorized exploitation of bio-resources?

Indian Patents Bill
Environment Protection Act
Biodiversity Act
Forest Conservation Act
3

The Biodiversity Act in India helps prevent the unauthorized exploitation of bio-resources.

Which plant hormone is essential for cell division and is used in tissue culture media?

Gibberellin
Cytokinin
Ethylene
Abscisic acid
2

Cytokinin promotes cell division and is an essential component of tissue culture media.

Which enzyme is used to join DNA fragments during recombinant DNA formation?

DNA polymerase
Ligase
Endonuclease
Reverse transcriptase
2

Ligase is used to join DNA fragments by forming phosphodiester bonds between them during recombinant DNA formation.

Which genetically modified plant produces beta-carotene to prevent vitamin A deficiency?

Bt cotton
Golden rice
Flavr Savr tomato
Hybrid maize
2

Golden rice is genetically modified to produce beta-carotene, which helps prevent vitamin A deficiency.

Which of the following is correct about plasmids used in genetic engineering?

Plasmids are linear DNA molecules.
Plasmids replicate independently within host cells.
Plasmids are never used as vectors.
Plasmids are found only in eukaryotic cells.
2

Plasmids are circular DNA molecules that replicate independently within host cells and are often used as vectors in genetic engineering.

Which of the following is correct about tissue culture?

Tissue culture produces genetically identical plants.
Tissue culture is used only for animal cells.
Tissue culture cannot produce whole plants from a single cell.
Tissue culture is not used in plant biotechnology.
1

Tissue culture is used to produce genetically identical plants through micropropagation.

Which of the following is correct about plasmid vectors used in genetic engineering?

Plasmid vectors cannot replicate independently.
Plasmid vectors are used to transfer foreign genes into host cells.
Plasmid vectors degrade DNA in host cells.
Plasmid vectors are only used in animal cell culture.
2

Plasmid vectors are used in genetic engineering to transfer foreign genes into host cells, where they replicate independently.

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