Biotechnology and its Applications Chapter-Wise Test 7

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following is incorrect about recombinant DNA technology?

Recombinant DNA is formed by combining DNA from different sources.
Recombinant DNA technology is used for gene cloning.
Recombinant DNA technology involves protein folding.
Recombinant DNA technology is used to produce insulin.
3

Recombinant DNA technology involves gene manipulation, not protein folding.

Which recombinant DNA product is used for treating growth hormone deficiency?

Insulin
Somatotropin
Interferon
Thyroxine
2

Recombinant human growth hormone (somatotropin) is used to treat growth hormone deficiency.

Which molecular diagnostic technique uses hybridization with radioactive probes?

PCR
ELISA
Autoradiography
Microarray
3

Autoradiography uses radioactive probes to detect specific DNA sequences by hybridization.

Which genetically modified crop is resistant to Lepidopteran pests?

Bt cotton
Golden rice
Tomato
Soybean
1

Bt cotton is genetically modified to resist Lepidopteran pests like bollworms.

Which technique uses RNA interference to silence specific gene expression in pests?

Micropropagation
Gene editing
RNA interference (RNAi)
Somatic hybridization
3

RNA interference (RNAi) is used to silence specific gene expression, including in pests for crop protection.

Which technique is used to separate and visualize DNA fragments?

ELISA
PCR
Gel electrophoresis
Northern blotting
3

Gel electrophoresis is used to separate and visualize DNA fragments based on size.

Which gene in Bt cotton provides resistance to bollworms?

cryIAc
cryIAb
cryIIAb
cryIIIa
1

The cryIAc gene from Bacillus thuringiensis provides resistance to cotton bollworms in Bt cotton.

Which of the following is incorrect about the Genetic Engineering Approval Committee (GEAC)?

GEAC regulates the use of genetically modified organisms in India.
GEAC ensures the safety of GMOs for the environment.
GEAC approves genetically engineered products for commercial use.
GEAC regulates traditional plant breeding methods.
4

GEAC does not regulate traditional plant breeding methods; it regulates the approval and safety of genetically modified organisms in India.

Which bacterial species is used for the transfer of genes into plants in biotechnology?

Bacillus thuringiensis
Agrobacterium tumefaciens
Escherichia coli
Pseudomonas fluorescens
2

Agrobacterium tumefaciens is used for transferring genes into plants in biotechnology.

Which of the following is incorrect regarding cry genes?

Cry genes encode proteins that are toxic to insect larvae.
Cry genes are obtained from Bacillus thuringiensis.
Cry proteins are produced during the log phase of bacterial growth.
Cry proteins form crystals that disrupt the insect gut.
3

Cry proteins are produced during the stationary phase of bacterial growth, not the log phase.

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