Biotechnology and its Applications Chapter-Wise Test 9

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which biotechnological method is used to introduce foreign DNA into animal cells?

Micropropagation
Microinjection
Somatic hybridization
Gene mapping
2

Microinjection is a method used to introduce foreign DNA directly into animal cells using fine glass needles.

Which nematode infects tobacco plant roots and is controlled by RNAi?

Meloidogyne incognita
Heterodera avenae
Caenorhabditis elegans
Ancylostoma duodenale
1

Meloidogyne incognita is a nematode that infects tobacco roots and is controlled using RNAi technology.

Which process involves the fusion of isolated protoplasts to form hybrid cells?

Gene editing
Somatic hybridization
Micropropagation
Mutation breeding
2

Somatic hybridization involves the fusion of isolated protoplasts from different plant species to form hybrid cells.

Which method is used to insert genes into plant cells using Agrobacterium?

Gene gun
Electroporation
Microinjection
Ti plasmid vector
4

Ti plasmid vector from Agrobacterium tumefaciens is used to insert genes into plant cells.

Which of the following is incorrect about plasmids?

Plasmids are circular DNA molecules.
Plasmids replicate independently within host cells.
Plasmids are used as vectors in genetic engineering.
Plasmids are part of the eukaryotic chromosome.
4

Plasmids are not part of the eukaryotic chromosome; they are extra-chromosomal circular DNA found primarily in bacteria and used as vectors in genetic engineering.

Which of the following statements about Golden Rice is correct?

Golden Rice is genetically modified for higher protein content.
Golden Rice is genetically modified for drought resistance.
Golden Rice produces beta-carotene to combat vitamin A deficiency.
Golden Rice is used as an insect-resistant crop.
3

Golden Rice is genetically modified to produce beta-carotene, which helps combat vitamin A deficiency.

Which Indian crop faced biopiracy when patented by an American company in 1997?

Wheat
Basmati rice
Sugarcane
Tea
2

Basmati rice faced biopiracy when an American company patented it in 1997.

Which of the following is correct about the Genetic Engineering Approval Committee (GEAC)?

GEAC regulates the use of GMOs in India.
GEAC approves traditional plant breeding methods.
GEAC monitors the use of antibiotics in hospitals.
GEAC is responsible for conducting genetic research.
1

The Genetic Engineering Approval Committee (GEAC) regulates the use, approval, and safety of genetically modified organisms (GMOs) in India.

Which of the following is incorrect about DNA ligase?

DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase is used in recombinant DNA technology.
DNA ligase breaks DNA strands at specific sites.
DNA ligase is essential in DNA replication and repair.
3

DNA ligase does not break DNA strands; it joins DNA fragments by forming phosphodiester bonds.

What is the primary role of cry genes in genetically modified crops?

Enhancing crop yield
Providing pest resistance
Improving nutritional value
Increasing drought tolerance
2

Cry genes produce proteins toxic to insect pests, providing pest resistance in genetically modified crops.

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